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KMID : 0385520080210010034
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Analytical Science & Technology
2008 Volume.21 No. 1 p.34 ~ p.40
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Determination of lercanidipine in human plasma by LC-MS/MS
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Jang Moon-Sun
La Soo-Kie
Chang Kyu-Young
Kang Seong-Woo
Han Sang-Beom
Lee Kyung-Ryul
Lee Hee-Joo
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Abstract
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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of lercanidipine in human plasma. After additionof internal standard (amlodipine), plasma was precipitated with acetonitrile and the supernatant was evaporated. The residues were dissolved in 50 % acetonitrile and analyzed by LC-MS/MS. Using MS/MS with multiple reaction monitoring (MRM) mode, lercanindipine were selectively detected without severe interference from human plasma. The standard calibration curve for lercanidipine was linear (r = 0.9994) over the concentration range 0.05-20.0 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of lercanidipine was lower than 11.7 % (correlation of variance, CV), and accuracy was between 94.4-114.8 %. This method has been successfully applied to the pharmacokinetic study of lercanidipine in human plasma.
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KEYWORD
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lercanidipine, LC-MS/MS, pharmacokinetic study
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